Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.136 extracted from

  • Mine, S.; Niiyama, M.; Hashimoto, W.; Ikegami, T.; Koma, D.; Ohmoto, T.; Fukuda, Y.; Inoue, T.; Abe, Y.; Ueda, T.; Morita, J.; Uegaki, K.; Nakamura, T.
    Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N-diacetylchitobiose deacetylase (2014), FEBS J., 281, 2584-2596 .
    View publication on PubMed
Search for more literature for 3.5.1.136

Cloned(Commentary)

Cloned (Comment) Organism
no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein Pyrococcus horikoshii
no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method, the crystal structure is determined at resolutions of 2.0 A Pyrococcus horikoshii
the crystal structure is determined at resolutions of 1.54 A Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
D46A complete loss of activity Pyrococcus horikoshii
H264A complete loss of activity Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the catalytic Zn2+ is situated at the end of the intermolecular cleft (which is formed by two polypeptides in the trimeric assembly) is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O Pyrococcus furiosus
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O Pyrococcus furiosus ATCC 43587
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii ATCC 700860
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3V1
-
-
Pyrococcus furiosus ATCC 43587 Q8U3V1
-
-
Pyrococcus horikoshii O58235
-
-
Pyrococcus horikoshii ATCC 700860 O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus furiosus beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus furiosus ATCC 43587 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Subunits

Subunits Comment Organism
hexamer composed of two trimers Pyrococcus horikoshii
hexamer composed of two trimers Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Dac
-
 Pyrococcus horikoshii
Dac
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 thermostable up to Pyrococcus horikoshii
85
-
 thermostable up to Pyrococcus furiosus